development of a new dna extraction protocol for pfge typing of mycobacterium tuberculosis complex.

نویسندگان

arash ghodousi department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran.

s vatani adept. of microbiology, school of medicine & infectious and tropical diseases research centre, ahvaz jundishapur university of medical sciences, ahvaz, iran..

davood darban-sarokhalil department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran.

maryam omrani department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran.

چکیده

a modified pulsed-field gel electrophoresis (pfge) protocol was developed and applied to clinical isolates of mycobacterium tuberculosis complex to reduce the cost of using lyticase. this protocol reduces the expense of pfge typing of mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

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عنوان ژورنال:
iranian journal of microbiology

جلد ۴، شماره ۱، صفحات ۴۴-۴۶

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